Permit and Opening Portion Free Essays

For this activity, please construct a series of questions that you would ask In the opening portion of the following types of interviews: 1 . To obtain information from a county official about building permits for a report you have been assigned to deliver to senior management 2. To write a biography of a long-time employee for a special presentation at her retirement party 3. We will write a custom essay sample on Permit and Opening Portion or any similar topic only for you Order Now To counsel a subordinate about a problem he or she is having keeping his or her business expenses within budget deadlines Activity 1 . Construct a series of questions that you would ask in the opening portion of the following types of interviews: a. To obtain information from a county official about building permits for a report you have been assigned to deliver to senior management What will we need for the permission to begin our project In this area? How long does It take to get the permit after we submit everything? Do we have an allotted time to finish the project, or do we have indefinite time to complete this? B. To write a biography of a long-time employee for a special presentation at her detriment party Brenda Mason, the dedication of a woman who has been working to supply for her children all these years, now gets the opportunity to celebrate her retirement and we are thankfully here to celebrate with her. She started working here 35 years ago, when her kids were Just 2 and 3, with the dream of becoming a decanter admit, and all the experience she ever had was witnessing. Her hard work got her into the entry level tech department with all the guys, and everyone treated her as she were a kindergarten, helped her with all the basics. She reemploy became the go-to to other people, and she was known for her charisma, and drive, we even tried to convince her to go to management, we wanted more people like her. She declined, and with that, still holding a positive attitude, she’d decline and said management was only â€Å"baby sitting adults†, she wanted to do a man’s job, she stuck to her dream. With absolute certainty we all knew she was going to be someone, with her two kids, and her job, working an additional 20 hours a week when her daughters began school, she got ahead of the whole department, raced the est. sales people, and learned all the server administrative tasks she ever could. She was getting to her dream, she applied to server support, and kept narrowing down her dream, spiraled Into It, till 3 years later, faster than anyone, she got It. Unbounded to us, this whole time, working the additional 20 hours, and she was In school, after the 3rd year she’d graduated, and qualified for those positions she qualified for. From there to now, she has brought that girl feel every tech department needed, the nagging and the whining, that got us all off our seat to get to work. We absolutely love her, and wish her the best, and to give her time to use up all the paid time off she earned. Equines expenses within budget guidelines We’ve noticed a few draw backs in your business, is everything okay with you and your family? We need to make sure you succeed here for them, if there is anything you need to do we will list it, but we are going over the goals you have failed to med, and re-structure the plan. You will report to us, the customers need to be served the right portions, you are busy, you have clientele, but you are allowing your employees to run your business and they re handing things off to their friends and family. This is coming out of your family resources, they depend on you, and you need to make sure that everyone is accountable for their actions. Your employees must get their receipts reviewed every night, and if I were you, I’d be having their submitted orders reviewed before the client checks out for the moment in order to have them understand how important this is. If they aren’t willing to comply, you have the right to fire them, you have to feed your family, they are not the right fit for the restaurant if that is the way they are behaving while they are clocked in. How to cite Permit and Opening Portion, Papers

Being homeless is often defined as sleeping on th Essay Example For Students

Being homeless is often defined as sleeping on th Essay e streets. Although this is the most visible and severe form of homelessness, there are many other types of acute housing need. These include living in temporary accommodation, poor or overcrowded conditions, or being in mortgage arrears and under threat of re-possession. (Hope 1986) It is a symptom of many complex problems: mental illness, emotional instability, illiteracy, chronic substance abuse, unemployment, and, most basic of all, breakdown of the family structure. Anyone can become homeless and the reasons that force people into homelessness are many and varied. The leading cause, however, of homelessness in the United States is the inability of poor people to afford housing. Housing costs have risen significantly over the last decade, whi. The Homeless Population One of the most important issues that concern me today is homelessness and the every year homeless increase. This is an important issue because millions of people throughout the world have no place to sleep or no food to eat. These people constantly wonder the streets begging for food, money, and any thing else that the people have to offer. The homeless have no remorse the winter beacuse they cant find a warm place to go and they freeze to death. Every year the homeless population increases. There are two major trends responsible for the rapid growth of homelessness over the past 15-20 years. These two trends include the growing shortage of affordable rental housing and a simultaneous increase in poverty. How many people are homeless throughout the world? The number of homeless is made up of 58% African American, 29% Caucasian, 10% Hispanic, 2% Native American, 1% Asian. Who is to blame for homelessness? As much as I would like to blame someone else other than the homeless people, they have a lot to do with it. Most of the homeless are out on the streets . .. HOMELESSNESS Homelessness is a very large problem that America has come to face with. Millions of people, including children, families, babies, veterans, and the elderly live day by day without food, water or a roof over their head. People that are mentally ill also have to tough it out on the streets, which can be very confusing to them, and dangerous to us. This problem must be solved soon, because its not getting any better. People have not always had to suffer with homelessness. Too bad the problem has almost always existed, it had not reached a severe level until recently. With every war there has been a small trickle of homeless veterans to follow, but Not enough people see the whole picture when it comes to societys problems. If something hurts the society, it hurts the individual. For example, the problem of homelessness is not just the problem of those who are without residence. Their plight effects us all. Homelessness hurts the local businessman whose customers are frightened away by the homeless man living on the corner. It hurts those who have to commute to work via public transportation and must deal with .. . Homelessness INTRODUCTION Homelessness is a condition of people who lack regular access to adequate housing. As this condition becomes a growing problem in Canada people are forced to deal with the issues. Who are the homeless? They range from children to adults and even in some cases, families. Why are they homeless? Poverty, lack of jobs or well paying jobs, decline in Social Services, domestic violence, mental illness .. . the majority of the homeless within our society. What effects does being homeless have on members of the family? It contributes to many physical and mental health problems for both parents and their children. Homelessness is a world-wide issue, yet zeroing in on Canada, the majority of the homeless live on the streets of Toronto and Vancouver where they seek shelter anywhere from a park bench .ucde205e8e2a0ff1bf959ba225f2b75d6 , .ucde205e8e2a0ff1bf959ba225f2b75d6 .postImageUrl , .ucde205e8e2a0ff1bf959ba225f2b75d6 .centered-text-area { min-height: 80px; position: relative; } .ucde205e8e2a0ff1bf959ba225f2b75d6 , .ucde205e8e2a0ff1bf959ba225f2b75d6:hover , .ucde205e8e2a0ff1bf959ba225f2b75d6:visited , .ucde205e8e2a0ff1bf959ba225f2b75d6:active { border:0!important; } .ucde205e8e2a0ff1bf959ba225f2b75d6 .clearfix:after { content: ""; display: table; clear: both; } .ucde205e8e2a0ff1bf959ba225f2b75d6 { display: block; transition: background-color 250ms; webkit-transition: background-color 250ms; width: 100%; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #95A5A6; } .ucde205e8e2a0ff1bf959ba225f2b75d6:active , .ucde205e8e2a0ff1bf959ba225f2b75d6:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #2C3E50; } .ucde205e8e2a0ff1bf959ba225f2b75d6 .centered-text-area { width: 100%; position: relative ; } .ucde205e8e2a0ff1bf959ba225f2b75d6 .ctaText { border-bottom: 0 solid #fff; color: #2980B9; font-size: 16px; font-weight: bold; margin: 0; padding: 0; text-decoration: underline; } .ucde205e8e2a0ff1bf959ba225f2b75d6 .postTitle { color: #FFFFFF; font-size: 16px; font-weight: 600; margin: 0; padding: 0; width: 100%; } .ucde205e8e2a0ff1bf959ba225f2b75d6 .ctaButton { background-color: #7F8C8D!important; color: #2980B9; border: none; border-radius: 3px; box-shadow: none; font-size: 14px; font-weight: bold; line-height: 26px; moz-border-radius: 3px; text-align: center; text-decoration: none; text-shadow: none; width: 80px; min-height: 80px; background: url(https://artscolumbia.org/wp-content/plugins/intelly-related-posts/assets/images/simple-arrow.png)no-repeat; position: absolute; right: 0; top: 0; } .ucde205e8e2a0ff1bf959ba225f2b75d6:hover .ctaButton { background-color: #34495E!important; } .ucde205e8e2a0ff1bf959ba225f2b75d6 .centered-text { display: table; height: 80px; padding-left : 18px; top: 0; } .ucde205e8e2a0ff1bf959ba225f2b75d6 .ucde205e8e2a0ff1bf959ba225f2b75d6-content { display: table-cell; margin: 0; padding: 0; padding-right: 108px; position: relative; vertical-align: middle; width: 100%; } .ucde205e8e2a0ff1bf959ba225f2b75d6:after { content: ""; display: block; clear: both; } READ: Genocide Essay Homeless What has been done to decrease the problem? One of the largest growing concerns in Toronto is the constantly increasing number of citizens who are finding themselves living on the streets. With the decrease in the number of available jobs, the population of homeless people has literally boomed. My questions are not as simple to answer as they may appear. Why is a large portion of our community forced to live on the streets? What has be done to decrease the problem? These are the questions I will confront in my essay. With the economical wealth attributed to the name .

Harverd Referencing System free essay sample

THE HARVARD REFERENCING SYSTEM The primary reason for correct referencing is to demonstrate what is your own work and what is work produced by other writers. Failure to quote your sources could lead to accusations of PLAGIARISM!! Plagiarism is intellectual theft as you are claiming work is your own when, in fact, that work belongs to someone else. Books The order for a Harvard reference is:- †¢ Author name [upper case] †¢ Year of publication †¢ Title of book [italics, first word upper case, the remainder lower case] †¢ Edition [if not 1st] †¢ Place of publication †¢ Publisher Example CHILDS M [2005] â€Å"Reward management† London; Chartered Institute of Personnel and Development BACH S and SISSON K [2000] â€Å"Personnel management; a comprehensive guide to theory and practice† 3rd ed Oxford; Blackwell Business Journal Articles Example BLACK J and GREGERSEN H [2000] â€Å"High impact training: forging leaders for the global frontier† Human Resource Management Vol. 39 Nos 2 3 Summer pp173-184 McCALL A [2003] â€Å"100 best companies to work for 2003† The Sunday Times Supplement 2nd March BURGOYNE J [2001] â€Å"Tester of faith† People Management Vol. We will write a custom essay sample on Harverd Referencing System or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page 7 No. 4 22nd February pp33-34 Internet †¢ Author Publication date †¢ Title of document or webpage †¢ URL of webpage †¢ Date you accessed the document Example http://www. acas. org. uk/publications/pub-cop. html [accessed 26th July 2010] http://www. stokesjolly. com/RoamAlone. doc [accessed 23rd July 2010] Quoting in the text Examples Taylor [2002] argues that †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦.. Taylor and Brown [2002] argue that †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦.. In a recent report [Taylor and Brown 2006] suggest that †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦ Taylor [2006 p44] defines management as â€Å"the art of getting things done through people. † Evans [2007 pp456-458] listed the following benefits of good management:- So:- when quoting exactly, always include the page number. If you are quoting exactly the reference should not be more than five lines. Location and arrangement of references A full list of ALL references used in the text MUST appear at the end of your work [before appendices]. References must be listed in author family name alphabetical order and laid out as shown above. Internet references follow on from book/journal references in the order used in the text. REMEMBER – INCORRECT REFERENCING COSTS MARKS! PLAGIARISM GETS NO MARKS PLUS OTHER PENALTIES. IF IN DOUBT – ASK!! Richard Underwood

Mothers Day Quotes From Famous People

Mothers Day Quotes From Famous People Mothers Day is celebrated on the second Sunday in May every year. Your own mother may not be the perfect cook or the greatest homemaker. But she is your mother a woman like no other, and she deserves more than just a Happy Mothers Day. Here are some thoughtful Mothers Day quotes to make her day memorable. Read some of the following motherhood quotes to understand what it means to be a mother, and help you put into words how priceless she truly is. Charlotte Gray Becoming a mother makes you the mother of all children. From now on each wounded, abandoned, frightened child is yours. You live in the suffering mothers of every race and creed and weep with them. You long to comfort all who are desolate. George Eliot A mothers yearning feels the presence of the cherished child even in the degraded man. Napoleon Bonaparte Let France have good mothers, and she will have good sons. Elizabeth Barrett Browning Women know the way to rear up children (to be just). They know a simple, merry, tender knack of tying sashes, fitting baby-shoes, and stringing pretty words that make no sense. And kissing full sense into empty words. Elizabeth Stone Making the decision to have a child is momentous. It is to decide forever to have your heart go walking around outside your body. Aristotle Mothers are fonder than fathers of their children because they are more certain they are their own. Aeschylus On me the tempest falls. It does not make me tremble. O holy Mother Earth, O air and sun, behold me. I am wronged. Washington Irving A mother is the truest friend we have, when trials, heavy and sudden, fall upon us; when adversity takes the place of prosperity; when friends who rejoice with us in our sunshine, desert us when troubles thicken around us, still will she cling to us, and endeavor by her kind precepts and counsels to dissipate the clouds of darkness, and cause peace to return to our hearts. Abraham Lincoln All that I am or ever hope to be, I owe to my angel Mother. Henry Ward Beecher The mothers heart is the childs schoolroom. George Washington My mother was the most beautiful woman I ever saw. All I am I owe to my mother. I attribute all my success in life to the moral, intellectual and physical education I received from her. James Russell Lowell That best academy, a mothers knee.

Comparing the Law of the Republic of Azerbaijan on Diplomatic Service Essay

Comparing the Law of the Republic of Azerbaijan on Diplomatic Service and UK Diplomatic Service Regulations - Essay Example In this way, the major areas that will be discussed between these two code of laws revolve around the expectation of impartiality, receiving of gifts or other remunerations, the level to which the monitoring and/or implementation of successful agreements is performed, whether or not discrimination is a determinant compliments that requires elaboration and definition, the process and regulations regarding the acceptance of gifts, and the level and extent to which the sponsoring state is ultimately responsible for providing the needs and welfare of the employed individuals within the diplomatic services. One of the first and most blatant the differentials that is noted with regards to the law concerning the diplomatic services between Azerbaijan and the United Kingdom is with respect to the overall level of importance and time that the United Kingdom’s code of law gives towards the importance of impartiality. For instance, the United Kingdom specifies the following in DSR one se ction 3: â€Å"As a civil servant, you are appointed on merit on the basis of fair and open competition and are expected to carry out your role with dedication and a commitment to the Diplomatic Service and its core values: integrity, honesty, objectivity and impartiality† (Diplomatic Service Regulations, 2012, p. 5). Further section 3 of DSR 1 goes on to state that impartiality is defined as â€Å" acting solely according to the merits of the case and serving equally well Governments of different political persuasions (Ibid). Although there is not a specific section within the law governing the actions and expectations of the diplomatic service operating at the behest of Azerbaijan, article 4 subsection to denotes the following with regards to the expectation concerning proper behavior of diplomatic professionals: â€Å"Diplomatic service agencies may carry out other functions in accordance with the Constitution and laws of the Republic of Azerbaijan† (Law of the Rep ublic of Azerbaijan, 2012, p. 2). Once again, a clear level of divergence is noted with respect to the fact that the laws governing diplomatic expectations within Azerbaijan deviate quite a bit from the law governing diplomatic expectations within the United Kingdom. Serving based upon impartiality is a fundamental construct of the UK law whereas serving in strict adherence and accordance with existing Azeri law and the Constitution serves as the regulatory framework and concern for the Azeri consular services. Another noted differential is with regards to the level and extent to which British law defines appropriate behavior and expected outcomes is with regards to what the regulations specific concerning appropriate remuneration and/or the acceptance/receipt of gifts. Whereas the British law allows for the receipt and acceptance of certain low monetary value gifts, special favors, discounts, or benefits of any other variety are strictly prohibited from being enjoyed by the individ ual employed by the diplomatic services. Although this is not to say that the receipt of gifts and or any level of persuasion/coercion on the part of a third-party is not specifically frowned upon within the regulations of consular and diplomatic conduct that had been laid out within the aforementioned case, no such specification of regulations

Bioterrorism Assignment Example | Topics and Well Written Essays - 500 words

Bioterrorism - Assignment Example Bioterrorism is a deliberate attempt to discharge biological agents to spread illness and eventually death of the inhabitants of particular geographical area. These biological agents may be bacteria, viruses or their toxins, either in their natural form or in their modified forms, or with enhanced virulence developed by humans to bring disaster. These agents can spread into the environment through air, water or food. It is enormously complicated to differentiate and detect the presence or these agents as they do not display any immediate complaints and also may not show symptoms for days. Thus, they serve as weapons which are cost-effective, trouble-free to disseminate and are capable of creating an extensive fear (Web- Bioterrorism Overview). One of the most discussed action was noticed in September-October 2001 in USA where numerous cases of anthrax bust out. These cases were due to the deliberate attempt and extended through letters carrying the potential anthrax agent, letters were delivered to the offices of news media along with the office of U. S Congress. Receivers of the letters were victimized with anthrax. Later on when tests were performed the strains were labelled as from a domesticate source. This incidence has paved the way for bio-terrorism and has wagered the meaning of biodefence and biosecurity, as it is highly focused exploitation of biological techniques (Web- Bioterrorism Overview). Bioterrorism agents can be categorized on the basis of the intensity of ailments or fatal consequences they create. Three categories are enumerated on the basis of the risk they dispose. Category A encompasses agents with highest risk while Category C encompasses agents with up-and-coming terrorization for disease. Agents belonging to Category A include: Anthrax (Bacillus anthracis); Botulism (Clostridium botulinum toxin); The Plague (Yersinia pestis); Smallpox (Variola major); Tularemia (Francisella tularensis); Hemorrahagic fever (Web-

Urinalysis of Four Urine Samples

Urinalysis of Four Urine Samples Urinalysis Practical Background: As you have learned, the urinary system performs many vital functions in the body including: Regulating blood volume and pressure by regulating water excretion, Regulating plasma ion/solute concentrations by adjusting urine composition, Assisting blood pH stabilisation, Removing nitrogenous waste, Conserving water and important nutrients and Assisting the liver in detoxifying poisons. Therefore, analysing a sample of urine from a person can provide important information on the health of that person. Urinalysis can reveal diseases such as diabetes mellitus, urinary tract infections and renal (kidney) infections such as glomeronephritis and kidney stones (renal calculi). A medical professional may perform a urinalysis for several reasons: As a general health check-up, Diagnosing metabolic or systemic diseases that affect renal function (heart failure will lead to decreased blood flow to the kidneys, pre-eclampsia during pregnancy will lead to increased protein in the urine), Diagnosis of endocrine disorders e.g. infertility (low levels of FSH and/or LH), Diagnosis of urinary system disease, Monitoring of glucose levels in patients with diabetes, Testing for pregnancy (hCG levels secreted by the embryo), Screening for drug use. Urinalysis is a technique involving physical, chemical and microscopic analyses of a sample of urine. Physical parameters: Normal urine is a clear yellow colour due to the presence of uribilin. Abnormal urine may be dark orange, red or brown and cloudy in appearance. This can be due to the presence of red and/or white blood cells or pigments and may indicate a urinary tract or renal infection or disease, liver or gall bladder disease. Normal urine has a specific gravity of between 1.002 – 1.028 (this is a measure of the number of particles/solutes in the urine, its concentration). A urine sample that has an elevated specific gravity can indicate dehydration, diarrhea/vomiting, glucosuria, inappropriate ADH secretion. A diminished specific gravity may indicate such diseases as renal failure or pyelonephritis. Chemical parameters: The chemical analysis of urine is routinely performed using an inexpensive and relatively accurate dipstick test (Uristix from Bayer or other brands). The test uses a reagent-coated plastic stick that is placed or dipped into the urine sample. The reagent areas change colour according to the presence of glucose and/or protein. (a) Figure 1. Colour chart (a) for determination of glucose and/or protein.   Ã‚   The glucose test on the dipstick is based on a double sequential enzyme reaction. One enzyme, glucose oxidase, catalyses the formation of gluconic acid and hydrogen peroxide from the oxidation of glucose (if present in the urine). A second enzyme, peroxidase, catalyses the reaction of hydrogen peroxide with a potassium iodide chromogen to oxidise the chromogen to colours ranging from green to brown. Normal urine has less than 0.1% glucose concentration. The protein test on the dipstick is based on the protein-error-of-indicators principle. At a constant pH, the development of any green colour is due to the presence of protein. Colours range from yellow for ‘negative’ through yellow-green and green to green-blue for ‘positive’ reactions. Normal urine has a protein concentration of less than 100  µg/ml. Although the dipstick test is semi-quantitative, significantly more accurate levels of glucose and protein can be determined by other means. In this practical you will use a BCA Assay (discussed later) to quantify the amount of protein present in a sample of urine. Urinalysis may also include assaying for levels of ketones (an indicator of diabetic ketosis, fasting or starvation), blood cells (indicating infection or kidney stones), bilirubin (liver or gall bladder disease), drugs and many other substances. Microscopic parameters: The urine sample can also be analysed by a microscope, often after staining to reveal any pathogens such as bacteria, urine crystals, cells and/or mucous. The presence of any of these may indicate infection or disease and further medical investigation will provide a thorough diagnosis. Aim: The aim of this practical is to perform glucose and protein urinalysis techniques on five samples of ‘urine’ provided by five ‘patients’ and use this information to provide an initial diagnosis for each patient. Part One: Using Dipsticks To Provide A Qualitative Measure of Protein And/Or Glucose. Materials: 5 samples of urine labelled A – E (these will be required for Parts One and Two), 5 Uristix dipsticks. Method: Perform a basic physical analysis of the urine samples noting the colour and cloudiness of each sample: Urine A Urine B Urine C Urine D Urine E Colour Cloudiness Immerse a dipstick into each of the samples, wait 60 seconds and record your results using the colour chart in Figure 1 to determine if the sample contains glucose and/or protein or neither substance: Urine A Urine B Urine C Urine D Urine E Glucose Protein Ketones Negative Negative Negative Negative Strongly positive Blood Negative Negative Trace Negative Negative Part Two: Using A Commercial BCA Assay To Provide A Quantitative Measure of Protein. Background: The BCA Protein Assay exploits the chemical reduction of Cu2+ to Cu1+ by protein in an alkaline medium with the selective colorimetric detection of the cuprous cation (Cu1+) by bicinchoninic acid (BCA). The first step is the chelation of copper with protein in an alkaline environment to form a blue coloured complex. In this reaction, known as the biuret reaction, peptides containing three or more amino acid residues form a coloured chelate complex with cupric ions in an alkaline environment containing sodium potassium tartrate. Single amino acids and dipeptides do not give the biuret reaction, but tripeptides and larger polypeptides or proteins will react to produce the light blue to violet complex that absorbs light at 540 nm. In the second step of the colour development reaction, BCA, a highly sensitive and selective colorimetric detection reagent reacts with the Cu1+ that was formed in step 1. The purple-coloured reaction product is formed by the chelation of two molecules of BCA with one Cu1+. The BCA/Cu complex is water-soluble and exhibits a strong linear absorbance at 562 nm with increasing protein concentrations. The rate of BCA colour formation is dependent on the incubation temperature, the types of protein present in the sample and the relative amounts of reactive amino acids contained in the proteins. Figure 2. Reaction diagram for the bicinchoninic acid (BCA) protein assay. Materials: The 2 samples of urine from Part One that were positive for protein, Protein stock standard (BSA, bovine serum albumin) at 1mg/ml, 0.9% Saline (diluent) BCA (bicinchoninic acid) Working Reagent (labelled BCA WR), 6 Tubes for dilutions for the standard curve, 96 Well microtitre plate, P100, P200 P1000 pipettes tips, Marker pen, 37ËÅ ¡C Incubator, Microtitre plate reader set to read at a wavelength of 562 nm. Method: Set up the dilutions for your standard curve, Label your tubes as 1,2,3,4,5 6, Prepare your standards according to the table below: Dilution tube # Volume of diluent ( µl) Volume of BSA or from tube # ( µl) Final BSA (protein) concentration ( µg/ml) 1 0  µl 300  µl BSA 1000 2 250  µl 250  µl bsa 500 3 250  µl 250  µl Tube 2 250 4 250  µl 250  µl Tube 3 125 5 800  µl 200  µl Tube 4 25 6 Blank 250  µl 0 0 Label your microtitre plate so that you know which wells hold your standards and which contain your samples (perform in triplicate), Pipette 25  µl of each standard (in triplicate) and sample (in triplicate) to each well, Add 200  µl of the Working Reagent to each standard or sample and shake for 30 seconds, Cover the plate and incubate at 37ËÅ ¡C for 30 minutes, Cool the plate to room temperature, Measure the absorbances at 562 nm on a microtitre plate reader, insert the average values in the table below: Dilution tube # Average absorbance at 562 nm (add 3 values divide by 3) Subtract blank (Tube 6) from value in previous column Final BSA (protein) concentration ( µg/ml) 1 1000 2 500 3 250 4 125 5 25 6 Blank 0 Sample 1 Sample 2 Prepare your standard curve: use the corrected absorbance readings for standards 1 – 6 (in column 3 in the previous table) and plot them against the amount of BSA in each tube, Once you have plotted your standard curve, you can determine the protein concentration in your samples, enter this value into the table above.   The patient scenarios are outlined below. You now need to match the urine samples with their corresponding patient scenarios and include justification for your decisions in your practical report: Patient Scenarios: Kidney Stones: Nida is a 17 year old student. She arrives at her GP feeling nauseous, feverish with acute pain in her lower back. She is also passing large amounts of blood in her urine. Glucose Drink: Thomas has just started a new job but is feeling quite stressed has lost weight. He arrives to see his GP but has had to skip lunch so drinks a litre of cola to maintain his energy levels. Diabetic: Jenny is studying for her A levels. Recently she has been losing weight although she is eating much more than usual is always hungry. Athlete: Dave is a professional athlete requires a blood urine test before competing in his next event. His test results are negative for drugs but are positive for another substance. Nephrotic syndrome: Keely is a 20 year old student. She has been feeling very unwell for some time with general fatigue, listlessness, weight loss puffiness around her eyes ankles. Her urine is very sparse very dark in colour